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rabbit anti gap43  (Proteintech)


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    Proteintech rabbit anti gap43
    Rabbit Anti Gap43, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+gap43/GAP43+Antibody/pmc12994538-98-23-26
    Average 95 stars, based on 133 article reviews
    rabbit anti gap43 - by Bioz Stars, 2026-11
    95/100 stars

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    other:

    Article Title: Obacunone Promotes Functional Recovery After Spinal Cord Injury by Attenuating Neuroinflammation by Targeting the TLR4/MyD88/p38 MAPK Pathway
    Article Snippet: The primary antibodies included rabbit anti-iNOS (13120, Cell signaling technology, 1: 1000), rabbit anti-TNFα (IPB9396, Baijia, 1:1000), rabbit anti-IL-1β (IPB0002, Baijia, 1: 1000), rabbit anti-GAP43 (16971-1-AP, Proteintech, 1:1000), mouse anti- α-tubulin (11224-1-AP, Proteintech, 1:1000), mouse anti-GAPDH (ab307799, Abcam, 1:5000), rabbit anti-Cleaved-caspase3 (AF7022, Affinity, 1: 1000), rabbit antiBax (505992-Ig, Proteintech, 1: 1000), rabbit anti-TLR4 (A17436, ABclonal, 1: 1000), rabbit anti-p38 MAPK (T55600, ABmart, 1: 1000), rabbit anti-Phospho-p38 MAPK (Thr180/Tyr182) (TA4001, ABmart, 1: 1000), rabbit anti-MyD88 (YM8747, immunoway, 1: 1000).

    Article Title: Obacunone Promotes Functional Recovery After Spinal Cord Injury by Attenuating Neuroinflammation by Targeting the TLR4/MyD88/p38 MAPK Pathway
    Article Snippet: The primary antibodies included rabbit anti-iNOS (13120, Cell signaling technology, 1: 1000), rabbit anti-TNFα (IPB9396, Baijia, 1:1000), rabbit anti-IL-1β (IPB0002, Baijia, 1: 1000), rabbit anti-GAP43 (16971-1-AP, Proteintech, 1:1000), mouse anti- α-tubulin (11224-1-AP, Proteintech, 1:1000), mouse anti-GAPDH (ab307799, Abcam, 1:5000), rabbit anti-Cleaved-caspase3 (AF7022, Affinity, 1: 1000), rabbit anti-Bax (505992-Ig, Proteintech, 1: 1000), rabbit anti-TLR4 (A17436, ABclonal, 1: 1000), rabbit anti-p38 MAPK ( T55600 , ABmart, 1: 1000), rabbit anti-Phospho-p38 MAPK (Thr180/Tyr182) (TA4001, ABmart, 1: 1000), rabbit anti-MyD88 (YM8747, immunoway, 1: 1000).

    Immunostaining:

    Article Title: Chronic stress hinders sensory axon regeneration via impairing mitochondrial cristae and OXPHOS.
    Article Snippet: After dissecting the sciatic nerve, 1 to 2 μl of AAV-hSyn-mCherry (BrainCase Co., China), AAV-Cre-mCherry (BrainCase Co., China), rAAV-hSynhM4D(Gi)-mCherry (BrainCase Co., China), rAAV-shRNAMCU-GFP (Viral Vector Core, Shanghai Jiao Tong University), or rAAV-sgSOD1-sgCAT-sgSOD2 (Viral Vector Core, Shanghai Jiao Tong University) was injected using a beveled, pulled, mineral oil–filled glass needle attached to a 10-μl Hamilton syringe. .. The antibodies used in the study for immunostaining were as follows: chicken anti–glial fibrillary acidic protein (1:3000; Abcam, ab4674), rabbit anti-GR (1:2000; Invitrogen, PA1-511A), mouse anti-GR (1:2000; Invitrogen, MA1-510), rabbit anti- GLAST (1:1000; Invitrogen, MA5-38203), mouse anti-NeuN (1:1000; Proteintech, 66836-1-Ig), rabbit anti-Kir4.1 (1:1000; Alomone Labs, APC-035), mouse anti-MR (1:1000; Abcam, ab64457), rabbit anti-HSP60 (1:3000; Proteintech,15282-1-AP), mouse anti-GAPDH (1:5000; Proteintech, 60004-1-Ig), rabbit anti-TOM20 (1:3000; Proteintech, 11802-1-AP), rabbit anti-NNT (1:1000; Proteintech,13442-2-AP), rabbit anti-GAP43 (1:1000; Cell Signaling Technology, 8945), mouse anti-LDHA (1:1000; Proteintech, 66287), anti-goat Alexa Fluor 488 (1:1000; Jackson ImmunoResearch, AB_2340428), anti-rabbit Alexa Fluor 594 (1:1000; Jackson ImmunoResearch, AB_2340621), anti-chicken Alexa Fluor 594 (1:1000; Jackson ImmunoResearch, AB_2337391), antirabbit Alexa Fluor 594 (1:1000; Jackson ImmunoResearch, AB_2338059), anti-rabbit Alexa Fluor 647 (1:1000; Jackson ImmunoResearch, AB_2338072), and anti-mouse Alexa Fluor 488 (1:1000; Jackson ImmunoResearch, AB_2721889). .. The statistical analysis was carried out using GraphPad Prism 9 software.



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    A MYCN expression of the SH-SY5Y, NBLS and NLF parental neuroblastoma cell lines by Western blotting. B TrkC and V5-tag expression in parental neuroblastoma cell lines and pLX302/NTRK3 transfected cells. C Western blot analyses of phospho-TrkC (Tyr 516 ), phospho-ERK (Thr 202 /Tyr 204 ), phospho-PLCγ (Tyr 783 ) following stimulation of SH-SY5Y/TrkC, NBLS/TrkC, NLF/TrkC cells with 100 ng/ml of NT-3 for 10 min. GAPDH acted as loading control for Western blot experiments. D Representative images of phenotypic observation by light microscopy 5 days post NT-3 (100 ng/ml) stimulation in SH-SY5Y/TrkC, NLF/TrkC and NBLS/TrkC neuroblastoma cells. Magnification: X20; scalebar: 200 µm. E Quantification of neuronal differentiation following NT-3 (100 ng/ml) treatment for 5 days. ( n = 3). F Western blot analysis of <t>GAP43,</t> a neuronal differentiation marker, following stimulation of SH-SY5Y/TrkC cells with 100 ng/ml of NT-3 for 5 days. Vinculin acted as loading control. ( n = 3). G Quantification of mean fluorescence as a measure of cell proliferation at 72 h post NT-3 (100 ng/ml) stimulation using CyQuant assay. Data shown as mean ± SEM; n = 3 ( p < 0.05 = *, p < 0.001 = **, p < 0.0001 = ***).
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    A MYCN expression of the SH-SY5Y, NBLS and NLF parental neuroblastoma cell lines by Western blotting. B TrkC and V5-tag expression in parental neuroblastoma cell lines and pLX302/NTRK3 transfected cells. C Western blot analyses of phospho-TrkC (Tyr 516 ), phospho-ERK (Thr 202 /Tyr 204 ), phospho-PLCγ (Tyr 783 ) following stimulation of SH-SY5Y/TrkC, NBLS/TrkC, NLF/TrkC cells with 100 ng/ml of NT-3 for 10 min. GAPDH acted as loading control for Western blot experiments. D Representative images of phenotypic observation by light microscopy 5 days post NT-3 (100 ng/ml) stimulation in SH-SY5Y/TrkC, NLF/TrkC and NBLS/TrkC neuroblastoma cells. Magnification: X20; scalebar: 200 µm. E Quantification of neuronal differentiation following NT-3 (100 ng/ml) treatment for 5 days. ( n = 3). F Western blot analysis of <t>GAP43,</t> a neuronal differentiation marker, following stimulation of SH-SY5Y/TrkC cells with 100 ng/ml of NT-3 for 5 days. Vinculin acted as loading control. ( n = 3). G Quantification of mean fluorescence as a measure of cell proliferation at 72 h post NT-3 (100 ng/ml) stimulation using CyQuant assay. Data shown as mean ± SEM; n = 3 ( p < 0.05 = *, p < 0.001 = **, p < 0.0001 = ***).
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    A MYCN expression of the SH-SY5Y, NBLS and NLF parental neuroblastoma cell lines by Western blotting. B TrkC and V5-tag expression in parental neuroblastoma cell lines and pLX302/NTRK3 transfected cells. C Western blot analyses of phospho-TrkC (Tyr 516 ), phospho-ERK (Thr 202 /Tyr 204 ), phospho-PLCγ (Tyr 783 ) following stimulation of SH-SY5Y/TrkC, NBLS/TrkC, NLF/TrkC cells with 100 ng/ml of NT-3 for 10 min. GAPDH acted as loading control for Western blot experiments. D Representative images of phenotypic observation by light microscopy 5 days post NT-3 (100 ng/ml) stimulation in SH-SY5Y/TrkC, NLF/TrkC and NBLS/TrkC neuroblastoma cells. Magnification: X20; scalebar: 200 µm. E Quantification of neuronal differentiation following NT-3 (100 ng/ml) treatment for 5 days. ( n = 3). F Western blot analysis of <t>GAP43,</t> a neuronal differentiation marker, following stimulation of SH-SY5Y/TrkC cells with 100 ng/ml of NT-3 for 5 days. Vinculin acted as loading control. ( n = 3). G Quantification of mean fluorescence as a measure of cell proliferation at 72 h post NT-3 (100 ng/ml) stimulation using CyQuant assay. Data shown as mean ± SEM; n = 3 ( p < 0.05 = *, p < 0.001 = **, p < 0.0001 = ***).
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    Image Search Results


    A MYCN expression of the SH-SY5Y, NBLS and NLF parental neuroblastoma cell lines by Western blotting. B TrkC and V5-tag expression in parental neuroblastoma cell lines and pLX302/NTRK3 transfected cells. C Western blot analyses of phospho-TrkC (Tyr 516 ), phospho-ERK (Thr 202 /Tyr 204 ), phospho-PLCγ (Tyr 783 ) following stimulation of SH-SY5Y/TrkC, NBLS/TrkC, NLF/TrkC cells with 100 ng/ml of NT-3 for 10 min. GAPDH acted as loading control for Western blot experiments. D Representative images of phenotypic observation by light microscopy 5 days post NT-3 (100 ng/ml) stimulation in SH-SY5Y/TrkC, NLF/TrkC and NBLS/TrkC neuroblastoma cells. Magnification: X20; scalebar: 200 µm. E Quantification of neuronal differentiation following NT-3 (100 ng/ml) treatment for 5 days. ( n = 3). F Western blot analysis of GAP43, a neuronal differentiation marker, following stimulation of SH-SY5Y/TrkC cells with 100 ng/ml of NT-3 for 5 days. Vinculin acted as loading control. ( n = 3). G Quantification of mean fluorescence as a measure of cell proliferation at 72 h post NT-3 (100 ng/ml) stimulation using CyQuant assay. Data shown as mean ± SEM; n = 3 ( p < 0.05 = *, p < 0.001 = **, p < 0.0001 = ***).

    Journal: Cell Death Discovery

    Article Title: MYCN inhibits TrkC-mediated differentiation in neuroblastoma cells via disruption of the PKA signalling pathway

    doi: 10.1038/s41420-026-03024-y

    Figure Lengend Snippet: A MYCN expression of the SH-SY5Y, NBLS and NLF parental neuroblastoma cell lines by Western blotting. B TrkC and V5-tag expression in parental neuroblastoma cell lines and pLX302/NTRK3 transfected cells. C Western blot analyses of phospho-TrkC (Tyr 516 ), phospho-ERK (Thr 202 /Tyr 204 ), phospho-PLCγ (Tyr 783 ) following stimulation of SH-SY5Y/TrkC, NBLS/TrkC, NLF/TrkC cells with 100 ng/ml of NT-3 for 10 min. GAPDH acted as loading control for Western blot experiments. D Representative images of phenotypic observation by light microscopy 5 days post NT-3 (100 ng/ml) stimulation in SH-SY5Y/TrkC, NLF/TrkC and NBLS/TrkC neuroblastoma cells. Magnification: X20; scalebar: 200 µm. E Quantification of neuronal differentiation following NT-3 (100 ng/ml) treatment for 5 days. ( n = 3). F Western blot analysis of GAP43, a neuronal differentiation marker, following stimulation of SH-SY5Y/TrkC cells with 100 ng/ml of NT-3 for 5 days. Vinculin acted as loading control. ( n = 3). G Quantification of mean fluorescence as a measure of cell proliferation at 72 h post NT-3 (100 ng/ml) stimulation using CyQuant assay. Data shown as mean ± SEM; n = 3 ( p < 0.05 = *, p < 0.001 = **, p < 0.0001 = ***).

    Article Snippet: Other antibodies used include TrkC (C44H5) (#3376), Phospho-ERK (Thr 202 /Tyr 204 ) (#4370), Phospho-Akt (Ser 473 ) (#4060), Akt (#9272), Phospho-PKA C (Thr 197 ) (#4781), PKA C-α (#4782), Vinculin (#4650), CREB (#9197), Phospho-CREB (Ser 133 ) (#9191), GAP43 (#8945), Tyrosine hydroxylase (#2792), GSK-3β (#12456), and GAPDH (14C10) (#2118), from Cell Signalling Technology.

    Techniques: Expressing, Western Blot, Transfection, Control, Light Microscopy, Marker, Fluorescence, CyQUANT Assay